diff --git a/Lib/test/test_httpservers.py b/Lib/test/test_httpservers.py
index 5dcedc0..3f14360 100644
--- a/Lib/test/test_httpservers.py
+++ b/Lib/test/test_httpservers.py
@@ -313,6 +313,9 @@
         #constructs the path relative to the root directory of the HTTPServer
         response = self.request(self.tempdir_name + '/test')
         self.check_status_and_reason(response, 200, data=self.data)
+        # check for trailing "/" which should return 404. See Issue17324
+        response = self.request(self.tempdir_name + '/test/')
+        self.check_status_and_reason(response, 404)
         response = self.request(self.tempdir_name + '/')
         self.check_status_and_reason(response, 200)
         response = self.request(self.tempdir_name)