diff --git a/Lib/test/test_subprocess.py b/Lib/test/test_subprocess.py
index 1cebf6b..868f279 100644
--- a/Lib/test/test_subprocess.py
+++ b/Lib/test/test_subprocess.py
@@ -682,7 +682,6 @@
             # on adding even when the environment in exec is empty.
             # Gentoo sandboxes also force LD_PRELOAD and SANDBOX_* to exist.
             return ('VERSIONER' in n or '__CF' in n or  # MacOS
-                    '__PYVENV_LAUNCHER__' in n or # MacOS framework build
                     n == 'LD_PRELOAD' or n.startswith('SANDBOX') or # Gentoo
                     n == 'LC_CTYPE') # Locale coercion triggered
 
diff --git a/Lib/test/test_venv.py b/Lib/test/test_venv.py
index a3b78c4..b2794cd 100644
--- a/Lib/test/test_venv.py
+++ b/Lib/test/test_venv.py
@@ -391,6 +391,18 @@
         self.assertEqual(err, "".encode())
 
 
+    @unittest.skipUnless(sys.platform == 'darwin', 'only relevant on macOS')
+    def test_macos_env(self):
+        rmtree(self.env_dir)
+        builder = venv.EnvBuilder()
+        builder.create(self.env_dir)
+
+        envpy = os.path.join(os.path.realpath(self.env_dir),
+                             self.bindir, self.exe)
+        out, err = check_output([envpy, '-c',
+            'import os; print("__PYVENV_LAUNCHER__" in os.environ)'])
+        self.assertEqual(out.strip(), 'False'.encode())
+
 @requireVenvCreate
 class EnsurePipTest(BaseTest):
     """Test venv module installation of pip."""