diff --git a/Python/pylifecycle.c b/Python/pylifecycle.c
index d31a9c1..eeaf20b 100644
--- a/Python/pylifecycle.c
+++ b/Python/pylifecycle.c
@@ -1057,6 +1057,8 @@ pyinit_main_reconfigure(PyThreadState *tstate)
 static PyStatus
 init_interp_main(PyThreadState *tstate)
 {
+    extern void _PyThread_debug_deprecation(void);
+
     assert(!_PyErr_Occurred(tstate));
 
     PyStatus status;
@@ -1158,6 +1160,9 @@ init_interp_main(PyThreadState *tstate)
 #endif
     }
 
+    // Warn about PYTHONTHREADDEBUG deprecation
+    _PyThread_debug_deprecation();
+
     assert(!_PyErr_Occurred(tstate));
 
     return _PyStatus_OK();